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| Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério. |
| Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011 |
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| Körbes,Ana Paula; Droste,Annette. |
| Somatic embryogenesis is an efficient method for the production of target cells for soybean genetic transformation. However, this method still offers low percentages of plant regeneration, and perhaps is related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to identify a maturation medium that could contribute to the outcome of more efficient plant regeneration results. Embryogenic clusters, derived from cotyledons of immature seeds of the soybean cultivars Bragg and IAS5, were used as starting material for embryos development. Different maturation media were tested by using 6% maltose, 3% sucrose or 6% sucrose, combined with or without 25 g L-1 of the osmotic regulator polyethylene glycol... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; PEG-8000; Sucrose; Osmotic regulation. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000300003 |
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